METHOD OF PREPARING HEPATOCYTE-LIKE CELLS AND BILE DUCT-LIKE CELLS USING HUMAN MINOR SALIVARY GLAND EPITHELIAL STEM/PROGENITOR CELLS AND USE THEREOF

摘要

The present invention provides a method of preparing hepatocyte-like cells and bile duct-like cells using human minor salivary gland epithelial stem/progenitor cells, and a use thereof. In the method, epithelial stem/progenitor cells are isolated from a human minor salivary gland tissue and cultured, further cultured and amplified in an epithelial cell culture medium, and then induced to differentiate in a hepatocyte-inducing culture medium containing a growth factor and utilizing a planar culture system or a three-dimensional culture system to obtain hepatocyte-like cells and bile duct-like cells.