Melatonin promotes the BMP9-induced osteogenic differentiation of mesenchymal stem cells by activating the AMPK/β-catenin signalling pathway

摘要

Melatonin enhances BMP9-induced early osteogenic marker alkaline phosphatase (ALP) activity in C3H10T1/2 cells. AdBMP9 and AdGFP are effective in infecting C3H10T1/2 cells. Melatonin induces ALP activity in C3H10T1/2 cells. Melatonin cooperates with BMP9 to induce ALP activity in C3H10T1/2 cells. C3H10T1/2 cells were treated with different concentrations of melatonin. C3H10T1/2 cells were treated with melatonin (100 μM), AdBMP9 alone or melatonin combined with AdBMP9. Primary MSCs were treated with melatonin (100 μM), AdBMP9 alone or melatonin combined with AdBMP9. Compared with the control group, ^^  p p p