Extracellular vesicles derived from human Wharton’s jelly mesenchymal stem cells protect hippocampal neurons from oxidative stress and synapse damage induced by amyloid-β oligomers

摘要

Characterization of hMSC-EVs. Nanoparticle tracking analysis of hMSC-EVs indicates a mixed population of exosomes and microvesicles, comprising particles with diameters ranging between 30 and 750 nm and an average particle density of 1.7 ± 0.7 × 10 particles/cell (  = 3 EV preparations from independent cord donors). hMSC-EVs were positive for MSC markers CD73, CD90, CD105 and negative for HLA-DR, CD14, CD34, CD45, and CD146. CD63-positive exosomes isolated from the total population of hMSC-EVs by CD63-conjugated capture beads are positive for exosome-associated tetraspanins, CD9 and CD81. , Representative TEM images of hMSC-EVs. White arrows indicate exosomes; black arrows indicate microvesicles