Extracellular vesicles from mast cells induce mesenchymal transition in airway epithelial cells

摘要

Epithelial cells take up mast cell-derived EVs and undergo morphological changes. High-resolution fluorescence imaging of A549 cells at 2 h and 16 h that have taken up HMC-1-derived EVs (10 μg/ml). Labels: EV lipids (green, PKH67), cell membrane (red, Alexa Fluor 594), and nucleus (blue, Hoechst 33342). Morphological assessment of Coomassie brilliant blue-stained A549 cells after 24 h of EV treatment (30 μg/ml). Representative images are shown from two independent experiments