DNMT3A reads and connects histone H3K36me2 to DNA methylation

摘要

. (A) Scatterplots showing genome-wide correlation between H3K36me2 and 5mC (left), and H3K36me3 and 5mC (right) in MDA-MB-231 cell. The number of reads was counted in nonoverlapping 100-kb bins spanning the human genome. The correlation coefficient was calculated with Pearson correlation. (B) Aggregation plot showing the distribution patterns of 5mC, H3K36me2 and H3K36me3 in the indicated genomic regions. (C) Schema of DNMT3A2 domain architecture and biotinylated peptide pull-down assay of DNMT3A2 FL and PWWP domain with the indicated histone H3.1K36 and H3.3K36 peptides. (D) DNA methylation assay testing the stimulation of DNMT3A activity with the indicated histone peptides (H3.1 22-44aa) (** value < 0.01) (left, radioactivity), and at different concentrations (right, Michaelis-Menten graph analyses, MBD2b based)