XIAP Interaction with E2F1 and Sp1 via its BIR2 and BIR3 domains specific activated MMP2 to promote bladder cancer invasion

摘要

The schematic structure of XIAP domains. , The indicated cell extracts were subjected to Western Blot to determine the expression of XIAP, MMP9, pro-MMP2, and cleaved-MMP2 (activated-MMP2). β-Actin was used as the protein loading control. T24T (Nonsense/Vector), T24T (shXIAP/Vector), and T24T (shXIAP/ΔRING) cells were cultured in uncoated chambers or pre-coated Matrigel chambers for 24 h. The cells were then fixed and stained. Original magnification, ×100. Scale bars, 10 µm. The invasion and migration rates were quantified by counting the relative migratory (Transwell) and invasive cells in five random fields (  = 5) under a light microscope, then the cell numbers were normalized with the insert control according to the manufacturer’s instructions. The error bars show the mean ± SD of three independent experiments. The symbol (*) indicates a significant difference as compared with the vehicle control (  p