PDK1 promotes ovarian cancer metastasis by modulating tumor-mesothelial adhesion invasion and angiogenesis via α5β1 integrin and JNK/IL-8 signaling

摘要

Upper: Immunohistochemical staining of PDK1 in serous (i) and mucinous (ii) benign cystadenomas, mucinous (iii), clear cell (iv), endometrial (v) carcinomas, primary serous carcinomas (vi), and matched metastatic foci (vii, viii, and ix). The insets highlight regions with higher magnification. Lower left: Immunoscoring of PDK1 in primary carcinomas and corresponding metastatic foci. The staining score for replicate metastatic lesions was averaged and considered as one value for that case for statistical purposes. Lower right: qPCR analysis of PDK1 mRNA in ovarian cancer samples (tumor) and their corresponding normal counterparts (non-tumor). Kaplan–Meier overall (left panel) and disease-free (right panel) survival curves with respect to PDK1 expression (cutoff at mean). Protein expression of PDK1 in HOSE (HOSE 11-12 and HOSE 17-1) and ovarian cancer cell lines (SKOV-3, OVCA 420, OVCA 429, OVCA 433, OVCAR-3, ES-2, and A2780CP) determined via immunoblot analysis.