Off-target effect of the BMI1 inhibitor PTC596 drives epithelial-mesenchymal transition in glioblastoma multiforme

摘要

Representative images of GBM0811 cells treated for 6 days by DMSO, PTC596 (5 and 50 nM), and A1016 (5 and 50 nM). Scale bar: 2.5 mm. Quantification of cell viability in GBM0811 cells treated for 6 days by DMSO, PTC596 (5 and 50 nM) and A1016 (5 and 50 nM). GBM1205 cell growth assay upon acute treatment (7 days) with BMI1 inhibitors PTC596 and A1016. Top: scheme of the assay. Bottom: representative images at each time point. Scale bar: 2.5 mm. Size distribution of the spheres at day 50 from experiment in after PTC596 or A1016 treatments in comparison to DMSO-treated cells. Colony-forming assay after acute treatment (2 days) with BMI1 inhibitors and two passages of the GBM0410 cells. Top: scheme of the assay. Bottom: representative images at each time point. Scale bar: 2.5 mm. Quantification of the number of spheres after one and two passages in PTC596 (Top) or A1016 (Bottom) treated cells.