Antioxidant Xanthine Oxidase α-Amylase and α-Glucosidase Inhibitory Activities of Bioactive Compounds from Rumex crispus L. Root

摘要

The root of L. has been shown to possess anti-gout and anti-diabetic properties, but the compounds responsible for these pharmaceutical effects have not yet been reported. In this study, we aimed to isolate and purify active components from the root of , and to evaluate their anti-radical, anti-gout and anti-diabetic capacities. From the ethyl acetate (EtOAc) extract, two compounds, chrysophanol ( ) and physcion ( ), were isolated by column chromatography with an elution of hexane and EtOAc at a 9:1 ratio. Their structures were identified by spectrometric techniques including gas chromatography-mass spectrometry (GC-MS), electrospray ionization-mass spectrometry (ESI-MS), X-ray diffraction analyses and nuclear magnetic resonance (NMR). The results of bioassays indicated that ( ) showed stronger activities than ( ). For antioxidant activity, ( ) and ( ) exhibited remarkable DPPH radical scavenging capacity (IC = 9.8 and 12.1 µg/mL), which was about two times stronger than BHT (IC = 19.4 µg/mL). The anti-gout property of ( ) and ( ) were comparable to the positive control allopurinol, these compounds exerted strong inhibition against the activity of xanthine oxidase (IC = 36.4 and 45.0 µg/mL, respectively). In the anti-diabetic assay, ( ) and ( ) displayed considerable inhibitory ability on α-glucosidase, their IC values (IC = 20.1 and 18.9 µg/mL, respectively) were higher than that of standard acarbose (IC = 143.4 µg/mL). Findings of this study highlight that ( ) and ( ) may be promising agents to treat gout and diabetes, which may greatly contribute to the medicinal properties of root.