New Hydroxydecanoic Acid Derivatives Produced by an Endophytic Yeast Aureobasidium pullulans AJF1 from Flowers of Aconitum carmichaeli

摘要

Endophytes have been recognized as a source for structurally novel and biologically active secondary metabolites. Among the host plants for endophytes, some medicinal plants that produce pharmaceuticals have been reported to carry endophytes, which could also produce bioactive secondary metabolites. In this study, the medicinal plant was selected as a potential source for endophytes. An endophytic microorganism, AJF1, harbored in the flower of , was cultured on a large scale and extracted with an organic solvent. Extensive chemical investigation of the extracts resulted in isolation of three lipid type compounds ( – ), which were identified to be (3 ,5 )-3,5-dihydroxydecanoic acid ( ), (3 ,5 )-3-(((3 ,5 )-3,5-dihydroxydecanoyl)oxy)-5-hydroxydecanoic acid ( ), and (3 ,5 )-3-(((3 ,5 )-5-(((3 ,5 )-3,5-dihydroxydecanoyl)oxy)-3-hydroxydecanoyl)oxy)-5-hydroxydecanoic acid ( ) by chemical methods in combination with spectral analysis. Compounds and had new structures. Absolute configurations of the isolated compounds ( – ) were established using modified Mosher’s method together with analysis of NMR data for their acetonide derivatives. All the isolates ( – ) were evaluated for antibiotic activities against , , , and their cytotoxicities against MCF-7 cancer cells. Unfortunately, they showed low antibiotic activities and cytotoxic activities.