Dual RNA-Seq Unveils the Role of the Pseudomonas plecoglossicida fliA Gene in Pathogen-Host Interaction with Larimichthys crocea

摘要

In the present study, and were selected as a host-pathogen interaction model for teleosts and prokaryotic pathogens. Five shRNAs were designed and synthesized to silence the gene, all of which resulted in pronounced reductions in mRNA; the mutant strain with the best silencing efficiency of 92.16% was chosen for subsequent analysis. A significant decrease in motility, intracellular survival and escape was observed for the -RNAi strain of , whereby silencing of the gene led to a 30% decrease in mortality and a four-day delay in the onset of infection in . Moreover, silencing of resulted in a significant change in both the pathogen and host transcriptome in the spleens of infected . Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis of pathogen transcriptome data showed that silencing resulted in downregulation of 18 flagellum-related genes; KEGG analysis of host transcriptome data revealed that infection with the -RNAi strain caused upregulation of 47 and downregulation of 106 immune-related genes. These pathogen-host interactions might facilitate clearance of by , with a significant decrease in -RNAi strain virulence in .