High copy number and highly stable Escherichia coli–Bacillus subtilis shuttle plasmids based on pWB980

摘要

Plasmids constructions. The was deleted from pWB980, resulting plasmid pWB980-DB. It was integrated with the replication-originated region from pUC19 at four essential sites up- and down-stream the membrane-binding region BA3-1 and the replicase-coding gene