Fate and Biofilm Formation of Wild-Type and Pressure-Stressed Pathogens of Public Health Concern in Surface Water and on Abiotic Surfaces

摘要

Since the historic outbreak near Broad Street in London, which serves as cornerstone of modern epidemiology, infectious diseases spread in surface and sub-surface water has been a persisting public health challenge. The current study investigated persistence of wild-type and pressure-stressed , O157:H7, and non-typhoidal serovars in surface water stored aerobically for up to 28 days at 5, 25, and 37 °C. Additionally, biofilm formation of wild-type and pressure-stressed non-typhoidal serovars were monitored on surface of stainless steel and rubber coupons for 28 days at 25 and 37 °C. While exhibited a lower ( < 0.05) survival rate at 5 °C, relative to the two Gram-negative pathogens, at higher temperatures of 25 and 37 °C, all three pathogens exhibited similar ( ≥ 0.05) trends for survival in surface water. Both wild-type and pressure-stressed serovars in the vast majority of tested times, temperatures, and surfaces exhibited comparable ( ≥ 0.05) persistence and biofilm formation capability. Our study thus indicates the occurrence of contamination could lead to prolonged survival of these microorganisms in low-nutrient environments and highlights the need for preventive measures such as those articulated under Produce Safety Rule of the U.S. Food Safety Modernization Act.