非生物胁迫条件下施氏假单胞菌生物膜形成规律的研究

摘要

In order to systematically investigate the effects of abiotic stress factors on the growth and biofilm formation of Pseudomonas stutzeri A1501,we measured the variation patterns of growth and biofilm formation of A1501 under different NaCl concentrations,pH and temperature.The results revealed that above 3 environmental factors did influence the growth and the biofilm formation of A1501 strain with certain extent of regularity.The optimal growth condition of A1501 with those factors was 2 mmol/L NaCl,pH6.8,and 30℃,respectively.However,the biofilm formation reached the maximum in the stress condition of 0.6 mol/L NaCl,pH6.0,and 40℃ with 7.8-,7.0-or 2.0-fold increment compared to the optimal growth condition,respectively.Among the three factors,NaCl showed the most significant effect on the biofilm formation of A 1501.In a conclusion,under the tested conditions,the biofilm formation of A 1501 strain was negatively correlated with the growth of strain,i.e.,high salt,weak acid and high temperature were adverse to the growth of A1501,however,it stimulated the biofilm formation.%旨在系统研究非生物胁迫因素对固氮施氏假单胞菌A1501生物膜形成能力的影响,测定了不同NaCl浓度、pH值、温度等培养条件下A1501生长及其生物膜形成能力的变化规律.结果显示,上述3种环境因素均对A1501菌的生长及生物膜形成具有影响,且呈现出一定的规律性.NaCl浓度2 mmol/L、pH值6.8、温度30℃是A1501的最适生长条件,而在0.6 mol/L的NaCl、pH值6.0及温度40℃等胁迫条件下,A1501菌的生物膜形成量均达到最高,分别为最佳生长条件下的7.8、7.0和2.0倍.在测试的诸因素中,NaCl浓度对A1501生物膜形成影响最大.以上结果表明,生物膜的形成与菌体生长负相关,即高盐、弱酸、高温等条件不利于A1501的生长,但刺激了其生物膜的形成.