Identification of disordered metabolic networks in postpartum dairy cows withleft displacement of the abomasum through integrated metabolomics and pathwayanalyses

摘要

High-producing dairy cows are easily affected by left displacement of the abomasum (LDA)within 4 weeks postpartum. Although LDA is highly associated with metabolic disturbances,the related information on comprehensive metabolic changes, with the exception of someblood biochemical parameters, remains limited. In this study, the changes in plasmametabolites and in the metabolic profile of postpartum dairy cows with LDA wereinvestigated through liquid chromatography coupled with quadrupole time of flight massspectrometry (LC-Q/TOF-MS)-based metabolomics, and the metabolic networks related to LDAwere constructed through metabolomics pathway analysis (MetPA). An obvious change in themetabolic profile was reflected by significant variations in 68 plasma metabolites inpostpartum dairy cows with LDA, and these variations consequently altered 13 metabolicpathways (histidine metabolism, tyrosine metabolism, valine, leucine and isoleucinebiosynthesis, phenylalanine, tyrosine and tryptophan biosynthesis, arginine and prolinemetabolism, tryptophan metabolism, synthesis and degradation of ketone bodies, linoleicacid metabolism, arachidonic acid metabolism, citrate cycle, butanoate metabolism, vitaminB metabolism and pyrimidine metabolism). This study shows that the moredetailed information obtained by LC-Q/TOF-MS-based metabolomics and MetPA might contributeto a better understanding of the disordered metabolic networks in postpartum dairy cowswith LDA.