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Comparison of Medium Temperature and Length of Incubation for Detection of Vancomycin-Resistant Enterococcus

机译:检测耐万古霉素肠球菌的培养基温度和孵育时间的比较

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摘要

Campylobacter (Campy; BD Diagnostics, Sparks, MD), Spectra VRE (Remel, Lenexa, KS), and bile-esculin-azide-vancomycin (BEAV; Remel) agars were compared for their ability to detect vancomycin-resistant enterococci (VRE) in 750 stool specimens. The media were compared at 24 h and 48 h of incubation at 35°C and 42°C. When incubated for 24 h at 35°C, Campy was the most sensitive (97.8%) and specific (99.9%) but was comparable to Spectra, which has a sensitivity of 95.6% and a specificity of 99.1%, whereas BEAV was significantly less sensitive (90%) and specific (96.1%). Incubation at 42°C or extended incubation at 35°C for 48 h yielded no advantage over incubation at 35°C for 24 h.
机译:比较了弯曲杆菌(Campy; BD Diagnostics,Sparks,MD),Spectra VRE(Remel,Lenexa,KS)和胆汁-叠氮化物-万古霉素(BEAV; Remel)琼脂的检测万古霉素抗性肠球菌(VRE)的能力。在750个粪便标本中。在35℃和42℃下孵育24小时和48小时时比较培养基。在35°C下孵育24小时时,Campy是最敏感的(97.8%)和特异的(99.9%),但与Spectra相当,后者的灵敏度为95.6%,特异度为99.1%,而BEAV则明显更低敏感(90%)和特异(96.1%)。与在35°C孵育24小时相比,在42°C孵育或在35°C扩展孵育48小时没有优势。

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