DETECTION OF VANCOMYCIN-RESISTANT ENTEROCOCCUS AND DETECTION MEDIUM

摘要

PROBLEM TO BE SOLVED: To provide a process for the detection and classification of vancomycin-resistant enterococcus having vanA vanB, vanC gene-type resistance which cannot be detected and classified by conventional method. ;SOLUTION: Vancomycin-resistant enterococcus is detected by smearing the bacteria on the surface of a medium containing a glycopeptide-type antibiotic substance exhibiting constitutive resistance to vancomycin-resistant enterococcus and enabling the growth of vancomycin-resistant enterococcus and culturing the bacteria on a part of the smeared surface in the presence of a glycopeptide- type antibiotic substance exhibiting induced resistance to vancomycin-resistant enterococcus and culturing on the other part of the surface in the absence of the antibiotic substance. The vancomycin-resistant gene type of the vancomycin-resistant enterococcus is discriminated by the growing state of the bacteria cultured by the above method.;COPYRIGHT: (C)2000,JPO