Essential oils of Origanum compactum increase membrane permeability disturb cell membrane integrity and suppress quorum-sensing phenotype in bacteria

摘要

The aim of this study was to investigate antibacterial activity of essential oils collected at three phenological stages on and . The antibacterial activity was evaluated using the agar-well diffusion assay. The MIC and MBC values were determined using the micro-dilution assay. The investigation of the antibacterial action was carried out by the evaluation of the effect of essential oils on the antibacterial kinetic growth, the integrity of cell membrane and permeability of the cell membrane. The anti-quorum sensing activity was tested by the inhibition of the biofilm formation. The findings of this study showed that essential oil has potent antibacterial activities against The lowest inhibition value against was obtained with essential oil at the post-flowering stage (MIC = MBC = 0.0312% (v/v)). The antibacterial mechanisms of essential oils are related to the disturbing of the cell membrane integrity and the increasing of the membrane permeability, which leads to the leakage of genetic materials (DNA and RNA). Moreover, essential oils inhibited the formation of the biofilms, a phenotype that has been known to be quorum sensing regulated.