Advanced oxidation protein products induce microglia-mediated neuroinflammation via MAPKs-NF-κB signaling pathway and pyroptosis after secondary spinal cord injury

摘要

AOPPs and oxidative stress levels were increased in rats with spinal cord injury (SCI). The body weight of rats was measured at different time points. , DHE staining of spinal cord tissues in different groups. The comparison of oxidative stress levels between SCI group and SCI apocynin group at 3 days after SCI. Scale bar, 50 μm. ‑ The concentration of AOPPs in plasma and CSF was detected by chloramine-T method at different time points and in different groups on day 3 after SCI. Data are representative of at least 3 independent experiments and the values are presented as mean ± SEM,  = 4 per group, *  P h, Immunofluorescence staining showed the expression of AOPPs in spinal cord tissues at different time points. The comparison of the levels of AOPPs between SCI group and SCI apocynin group was performed on day 3 after SCI. Scale bar, 100 μm. Western blot analysis showed the contents of AOPPs in spinal cord in different groups on day 3 after SCI.  = 4 per group, *  P k The concentration of AOPPs in spinal cord was detected by chloramine-T method in different groups on day 3 after SCI. Data are representative of at least 3 independent experiments and the values are presented as mean ± SEM,  = 4 per group, *  P