Hydrophilic bile acids prevent liver damage caused by lack of biliary phospholipid in Mdr2−/− mice

摘要

Bile acid imbalance causes progressive familial intrahepatic cholestasis type 2 (PFIC2) or type 3 (PFIC3), severe liver diseases associated with genetic defects in the biliary bile acid transporter bile salt export pump (BSEP; ABCB11) or phosphatidylcholine transporter multidrug resistance protein 3 (MDR3; ABCB4), respectively. mice (a PFIC3 model) develop progressive cholangitis, ductular proliferation, periportal fibrosis, and hepatocellular carcinoma (HCC) because the nonmicelle-bound bile acids in the bile of these mice are toxic. We asked whether the highly hydrophilic bile acids generated by mice could protect mice from progressive liver damage. We generated double-KO (DKO: and ) mice. Their bile acid composition resembles that of mice, with increased hydrophilic muricholic acids, tetrahydroxylated bile acids (THBAs), and reduced hydrophobic cholic acid. These mice lack the liver pathology of their littermates. The livers of DKO mice have gene expression profiles very similar to mice, with 4,410 of 6,134 gene expression changes associated with the mutation being suppressed. Feeding with THBAs partially alleviates liver damage in the mice. Hydrophilic changes to biliary bile acid composition, including introduction of THBA, can prevent the progressive liver pathology associated with the (PFIC3) mutation.