Single-cell RNA sequencing reveals compartmental remodeling of tumor-infiltrating immune cells induced by anti-CD47 targeting in pancreatic cancer

摘要

Immunostaining of CD47, PD-L1, CD68, and CD163 in human PDAC. Staining with an anti-CD47, anti-PD-L1, anti-CD68, and anti-CD163 antibody in the human PDAC tissue samples at × 100 magnification and × 400 magnification. Scale bar = 50 μm (red line at the bottom left). Results of immunohistochemical staining. Immunoblotting of CD47 and PD-L1 in PDAC cell lines. ACTB (β-actin) was used as a normalization control. Kaplan–Meyer plot of OS in 106 PDAC patients with high or low tumor CD47 expression. Kaplan–Meyer plot of OS in 106 PDAC patients with high or low tumor PD-L1 expression. , Kaplan–Meyer plot of OS in 106 PDAC patients with high or low tumor-infiltrating CD68 ( ) or CD163 macrophages ( ). – Kaplan–Meyer plot of OS among four groups of patients divided on the combinations of two variables with high and low expression. is an illustration of – . The four groups are and in blue, and in gold, and in green, and and in red. “ ” and “ ” represent the two different variables (CD47, PD-L1, CD68 M, or CD163 M2). “ ” represent the numbers of patients in four groups (blue, gold, green, and red)