Ginseng-derived nanoparticles alter macrophage polarization to inhibit melanoma growth

摘要

Characterization of ginseng-derived nanoparticles (GDNPs) prepared from ginseng roots. Ginseng root juice was purified by sucrose density gradient (15%/30%/45%/60%) under ultracentrifugation, and the band from the interface of 45% (band 3) was harvested and defined as GDNPs according to the literature for further use. GDNPs harvested from the sucrose density gradient (45%) were characterized by transmission electron microscopy (TEM) (Scale bar = 500 nm). Particle size of the GDNPs was measured by dynamic light scattering (DLS). Representative chart of the surface charge of GDNPs determined by dynamic light scattering coupled with Laser Doppler Velocimetry. Ginsenoside Rg3 contents of GDNPs in each batch. Pie chart of the composition of GDNPs showing the percentage of total metabolites. The composition for each protein and metabolite molecular species is reported as % of total proteins and metabolites analysed. For each category, a two-tailed Fisher’s exact test was applied to test the enrichment of the differentially expressed protein against all identified proteins. These analyses are derived from two biological replicates