Overexpressed ITGA2 promotes malignant tumor aggression by up-regulating PD-L1 expression through the activation of the STAT3 signaling pathway

摘要

The overexpression of ITGA2 is correlated with unfavorable prognosis in malignant tumors. . ITGA2 mRNA expression level determined by the GEPIA web tool. The boxplot analysis showed the expression level by log2 (TPM + 1) on a log-scale. ns, not significant; *,  b. IHC Images of ITGA2 staining using TMA tissue sections. The scale bars were shown in the figure. . Dot plots to show the IHC score of ITGA2 expression using TMA tissue sections (normal pancreatic specimens:  = 25, PDAC TMA specimens:  = 31,  d and . Western blot analysis for the expression of ITGA2 in 12 paired primary PDAC tissues (T) and the matched adjacent normal tissues (ANT) of the same patient (d). The proteins expression level of ITGA2 were quantified by ImageJ software (e). GAPDH served as an internal reference. The values were also shown. Statistical analyses were performed with D’Agostino & Pearson omnibus normality test. and . GEPIA web tool was searched for the overall survival (f,  = 0.015, HR = 2.0) and disease-free survival ( ,  = 0.039, HR = 1.9) of PDAC patients. . The Human Protein Atlas database was searched for the overall survival of PDAC patients (