Histone Deubiquitinase OTU1 Epigenetically Regulates DA1 and DA2 Which Control Arabidopsis Seed and Organ Size

摘要

Seeds are central to plant life cycle and to human nutrition, functioning as the major supplier of human population energy intake. To understand better the roles of enzymic writers and erasers of the epigenetic marks, in particular, histone ubiquitylation and the corresponding histone modifiers, involved in control of seed development, we identified the otubain-like cysteine protease OTU1 as a histone deubiquitinase involved in transcriptional repression of the and genes known to regulate seed and organ size in . Loss-of-function mutants of accumulate H2B monoubiquitylation and such euchromatic marks as H3 trimethylation and hyperacetylation in the and chromatin. These data advance our knowledge about epigenetic regulation of the and genes by recognizing OTU1 as a member of a putative repressor complex that negatively regulates their transcription.