The ability to manipulate ROS metabolism in pepper may affect aphid virulence

摘要

Plants received clip cage with aphids of the NL (NL-treated) or SW (SW-treated) population, control plants received an empty clip cage. After a 6-h incubation, leaf disks were harvested and analyzed using RNAseq. The criteria used for assigning significance were: FDR ≤ 0.01 and |log2FoldChange| ≥ 1. Number of upregulated or downregulated DEGs; number of DEGs specifically or coexpressed in comparison between control and NL-treated plant as well as in comparison between control and SW-treated plant. Venn diagram of DEGs that specifically respond to the feeding by the NL or the SW population. DEGs were first compared between the NL-treated vs. control plants and SW-treated vs. control plants. One hundred and five genes were differently regulated in the comparison between NL-treated and control plants (yellow circle) and 368 genes were differently regulated in the comparison between SW-treated and control plants (red circle). The lists of 105 and 368 genes were then compared with the list of 690 DEGs that were detected in the direct comparison between NL-treated and SW-treated plants. The 63 and 203 genes that appeared in both comparisons were considered here to specifically respond to the feeding by the NL and the SW populations respectively.