Molecular Typing of a Suspected Cluster of Nocardia farcinica Infections by Use of Randomly Amplified Polymorphic DNA Pulsed-Field Gel Electrophoresis and Amplified Fragment Length Polymorphism Analyses

摘要

Randomly amplified polymorphic DNA (RAPD), pulsed-field gelelectrophoresis (PFGE), and amplified fragment length polymorphism (AFLP) analyses were used to investigate a possible outbreak of Nocardia farcinica. RAPD and PFGE analyses yielded irreproducible and unsatisfactory results, respectively. AFLP analysis seem to be a promising and welcome addition for molecular analysis of Nocardia isolates.