Comparison of two modified methods of intrathecal catheterization inrats

摘要

The study designed to compare two different methods of intrathecal catheterization inrats and to develop a simple and safe drug administration in cervical spinal canal ofrats. The subarachnoid catheterization was performed via either atlanto-occipital membraneor laminectomy at L3–4 in rats. Body weight, Basso, Beattie, and Bresnahan (BBB)locomotion rating scores and forelimb locomotor rating scale (FLS) were measured onpre-operative day 1 and postoperative day 1, 7, 14, respectively. FLS score of 37.5% ratsand BBB score of 50% rats in the atlanto-occipital approach (AOA) group decreased, but norats showed locomotor impairment in the lumber approach (LA) group. The mean body weightof rats in AOA group reduced significantly compared with LA group. In LA group, 62.5% ofcatheter tips were located at T1, and in AOA group, the tips of catheter located at C2 in62.5% cases. The PE10 catheter can be successfully inserted into the spinal intrathecalspace for chronic delivery of drugs either via L3–L4 interlaminar space or viaatlanto-occipital membrane. And the subarachnoid catheterization via L3–L4 interlaminarspace could be easily placed at T1 with little complication.