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Dataset of MAPLE Parameters for Hemoglobin Deposition upon long period gratings

机译:长期光栅上血红蛋白沉积的MAPLE参数数据集

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摘要

Matrix-assisted pulsed laser evaporation (MAPLE) is an alternative and complimentary method to pulsed laser deposition. MAPLE has been demonstrated to be a less harmful approach for transporting and depositing delicate, highly sensitive molecules. Metalloproteins are considered sensitive molecules since their bioactivity is determined not only by their chemical structure but also by conformational changes that can be altered by deposition methods. Here we report a dataset of MAPLE deposition parameters of haemoglobin (Hb) that ensures the retention of its bioactivity. Methods for parameters optimization are also described. The data and analysis should be valuable for researchers interested in application of MAPLE techniques for metalloprotein immobilization since it provides a unique opportunity for direct immobilization. The data presents the results of previously conducted experiments on the basis of which is based the research article entitled “A Highly Efficient Biosensor based on MAPLE Deposited Hemoglobin on LPGs Around Phase Matching Turning Point” .
机译:基质辅助脉冲激光蒸发(MAPLE)是脉冲激光沉积的替代和补充方法。已经证明,MAPLE是运输和沉积敏感的高度敏感分子的一种危害较小的方法。金属蛋白被认为是敏感分子,因为它们的生物活性不仅取决于其化学结构,还取决于可以通过沉积方法改变的构象变化。在这里,我们报告血红蛋白(Hb)的MAPLE沉积参数的数据集,以确保其生物活性的保留。还描述了参数优化的方法。数据和分析对于有兴趣将MAPLE技术应用于金属蛋白固定化的研究人员来说很有价值,因为它为直接固定化提供了独特的机会。数据提供了先前进行的实验的结果,其基础是名为“基于MAPLE沉积的血红蛋白在相匹配转折点附近的LPG上的高效生物传感器”的研究文章。

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