An abscisic acid-responsive protein interaction network for sucrose non-fermenting related kinase1 in abiotic stress response

摘要

Overview of the Y2H screen. Six SnRK1 subunits were fused to the GAL4 activation domain (AD) and mated with 254 ABA library clones fused to the LexA DNA binding domain (BD). Positive interactors were scored on solid media lacking leucine or supplemented with X-gal. Two replicate plates per reporter were conducted for each of the six SnRK1 subunits. Representation of the ABA-responsive SnRK1 interaction network. Forty-eight core SnRK1-interacting-proteins (SnIPs) partnered with SnRK1α and β/βγ subunits (nodes in the large central circle). Proteins (nodes) are colored based on GO molecular function. Y2H assay sensitivity and background determined with a positive (PRS) and random (RRS) reference set, respectively. One hundred and thirty two high confidence interactions (HCI) were retested by Y2H and eight by BiFC. GO Slim molecular function distribution of the 125 SnRK1 complex interacting proteins (SnIPs).