Tetrahydrobenzimidazole TMQ0153 triggers apoptosis autophagy and necroptosis crosstalk in chronic myeloid leukemia

摘要

Chemical structure of TMQ0153. Time- and dose-dependent effect of TMQ0153 on K562 cell proliferation (left panel) and viability (right panel). Inhibitory effect of increasing concentrations of TMQ0153 on the colony forming capacities of the indicated CML cell lines. Upper panel: pictures representative of three independent experiments. Lower panel: quantification of the colony numbers. All data represent mean (±S.D.) of three independent experiments. Acute toxicity assay on zebrafish embryos after 24 h of treatment with increasing concentrations of TMQ0153. Pictures are representative of 10 fishes used for each condition (left panel) and the corresponding quantification of viable embryos (right panel). Ethanol (EtOH, 70%) was used as a positive control for toxicity. Cytotoxicity of TMQ0153 on human peripheral blood mononuclear cells (PBMCs) by Trypan blue assay after 24, 48, and 72 h of treatment (PBMC data represent the mean (±S.D.) of five independent experiments). Statistical significance was assessed as *  p p