Yes-associated protein regulates podocyte cell cycle re-entry and dedifferentiation in adriamycin-induced nephropathy

摘要

Urinary albumin/creatinine ratio in mice of the control group and adriamycin-treated group; error bars indicate the SD (  = 3). Immuno- histochemical staining showed expression of PCNA in the glomerulus; podocytes are indicated by an arrow (magnification, ×200). In vitro experiments showed the expression of PCNA in podocytes after adriamycin (0.25 µg/mL) treatment for the indicated time (magnification, ×200). Schematic diagram of cell cycle detection results; the diagonal strip area represents the proportion of cells in S phase. The proportion of cells in S phase in different groups; data are from at least three replicates. – Real-time PCR detection of cell cycle-related gene expression after adriamycin treatment; data are from at least three replicates. Western blotting analysis of the expression of cell cycle-associated proteins and their inhibitors in podocytes treated with adriamycin (0.25 µg/mL) for the indicated time periods. Quantification of band density relative to the β-actin control (  = 3 per group). Immunohistochemical analysis showed that CDK4 and cyclin D1 were expressed in podocytes of adriamycin-treated mice; the arrow indicates the location of the podocyte and the magnification was ×400. control, Adriamycin, 4′,6-diamidino-2-phenylindole. *