Iron free zinc oxide nanoparticles with ion-leaking properties disrupt intracellular ROS and iron homeostasis to induce ferroptosis

摘要

HUVECs were treated with ZnO NPs (0, 5, and 10 μg/mL) for 24 h. GSH, GPx, ROS, and MDA levels were measured. Total RNA was extracted for the analysis of mRNA levels of interest using qRT-PCR. GPx4, ACSL4, ALOX15, and PTGS2 mRNA. HUVECs were pretreated with DFO (100 μM) for 1 h in the presence of 10 μg/mL ZnO NPs. The generation of ROS was determined by the DCFH-DA probe, MFI: mean fluorescence intensity. Cell viability analysis. LDH leakage assay. Data are shown as the mean ± S.D. from three independent experiments.