CCL5 derived from tumor-associated macrophages promotes prostate cancer stem cells and metastasis via activating β-catenin/STAT3 signaling

摘要

, The mRNA and protein expression differences of indicated genes between prostate cancer tumor tissues and para-carcinoma tissues (  = 3). Elisa assay indicated that the blood samples of prostate cancer patients (  = 30) exhibited elevated expression of CCL5 when compared with that of healthy male participants (  = 30). Prostate cancer patients with higher Gleason grade exhibited an increased CCL5 accumulation in the blood (  = 30). Tissue immunofluorescence assay suggested that CCL5 and the macrophage marker CD163 were co-localized in both the primary tumor and metastatic lymph node of prostate cancer patients. Scale bar, 10 μm. TAMs exhibited increased secretion of CCL5 than immature macrophages or prostate cancer cells. CCL5 concentrations in the cell culture supernatants of THP1-derived macrophages (M0), THP1-derived TAMs (M2), DU145 and PC3 cells were measured using Elisa method (  = 10). All values are presented as the mean ± SD. *  p