TCF 4 tumor suppressor: a molecular target in the prognosis of sporadic colorectal cancer in humans

摘要

Mutation detection in exon 1 of TCF-4 gene by PCR amplification and SSCP analysis. shows agarose gel electrophoresis of PCR product of exon 1 of TCF-4 gene from 2 representative cases of CRC (N: normal tissue, A: adjoining tissue, T: tumor tissue). MW: molecular weight marker (100 bp DNA ladder). Arrow represents the amplified PCR product of 234 bp. shows SSCP analysis of PCR product of exon 1 of TCF-4 gene. Gel picture shows the representative image of the three samples that demonstrates the band shift. Arrow represents the bands showing mobility shift in tumor tissues compared to adjoining and normal tissues. shows the percentage of mutations in exon 1 of TCF-4 gene in tumor tissues compared to adjoining and normal tissue