Correction to: Inhibition of the key metabolic pathways glycolysis and lipogenesis of oral cancer by bitter melon extract

摘要

BME treatment reduces expression of glycolytic genes. Relative mRNA expression of GLUT-1, PFKP, PKM, LDHA, and PDK3 was analysed by q-RT-PCR in Cal27 and JHU022 cells with/without BME. 18 s gene was used as internal control. Cell lysates from Cal27 and JHU022 with or without BME treatment for 30 h were subjected to Western blot analysis for GLUT-1, PFKP, LDHA, PKM and PDK3 using specific antibodies. The membrane was reprobed with antibody to actin as an internal control. Quantitative of Western blot band intensities using Image-J software. Small bar indicates standard error (*,  p p d: Schematic diagram showing different genes regulate glycolysis and effect of BME on the genes