Ubiquitin ligase CHAF1B induces cisplatin resistance in lung adenocarcinoma by promoting NCOR2 degradation

摘要

High CHAF1B expression was negatively correlated with prognosis of LUAD patients and regulated cisplatin sensitivity. Whole proteome chips showed chat there are 657 significantly changed protein, of which 312 significantly up-regulated protein, and 345 significantly down-regulated protein. There are 46 significantly up-regulated ubiquitination enzymes, including 42 E3; According to the public database, 8 E3 high expressions, including: ARPC1A, AURKA, CDC20, CDCA3, CHAF1B, FBXO22, PPP1R13L, TRIP12, were negatively correlated with the prognosis of patients with lung adenocarcinoma; PCR results indicated that the expression of CHAF1B, PPP1R13L and CDC20 in A549/DDP was significantly higher than that in A549; d After knocking down CHAF1B, PPP1R13L and CDC20, the proliferation of A549/DDP cells was significantly decreased, and IC50 was significantly down-regulated; e CHAF1B substrate screening. * p