Laparoscopic Surgery Versus Open Surgery for Colorectal Cancer:Impacts on Natural Killer Cells

摘要

Laparoscopic resection is increasingly used incolorectal cancer (CRC). It has been suggested to carry short-term benefits insafety, recovery, and preservation on immune function for patients with CRC.However, the impact of laparoscopic resection on natural killer (NK) cells islargely unclear. Methods: A total of 200 patients with CRC across Dukes A/B/Cstages were randomly assigned to laparoscopic or open resection. The bloodsamples were collected before and after the surgery. The total number of NKcells was quantified by flow cytometer. Lytic units 35 toward K562 was used toquantify NK cells activity. The outcomes between the groups across pathologicalstages were also analyzed. Results: The number and activity of NK cellsdecreased after the surgery in both groups. The laparoscopic group showed afaster recovery rate of NK cells function than the control group as assessed bycell count and lytic activity. Natural killer cells were impaired in a higherdegree in patients at Dukes B/C stages. The recovery of NK cells to baselinelevel at day 7 postsurgery was observed in the laparoscopic group across all 3stages. Conclusion: Generally, laparoscopically assisted surgery resulted in abetter preservation on NK cells function. A better outcome was observed inpatients with CRC at Dukes B/C stages.