Differential effects of MLO-A5 osteocyte-derived conditioned medium (A5 CM) with and without fluid flow (FF) treatment at 1 Pa on TMD cells. CN control and A5 A5 CM. The single and double asterisks indicate P a Representative images of A5 and Y4 cells and the expression of Sclerostin (Scl) and DMP1. Inhibition of 2-dimensional cellular migration in a scratch assay by A5 CM. Stimulation of cell proliferation by A5 CM in an EdU assay. Compaction of TMD tumor spheroids by A5 CM. Downregulation of Vimentin (Vim), MMP9, Snail, and Slug and upregulation of E-cadherin (E-cad) and p-Akt by A5 CM. Promotion of cellular migration in a scratch assay by A5 FFCM. Reduction in cellular proliferation by A5 FFCM. Expansion of TMD tumor spheroids by A5 FFCM. Upregulation of Vimentin, MMP9, Snail, and Slug and downregulation of E-cadherin and p-Akt by A5 FFCM
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