Auxin protects Arabidopsis thaliana cell suspension cultures from programmed cell death induced by the cellulose biosynthesis inhibitors thaxtomin A and isoxaben

摘要

Calcium influx is required for the CBI-induction of cell death. Percentage of dead cells in suspension cells 48 h after the addition of calcium transport inhibitors. and Cells were treated with thaxtomin A (TA; 1 μM), isoxaben (IXB; 1 μM) or lanthanum chloride (LaCl ; 500 μM) alone, or pretreated with LaCl (500 μM) 30 min before adding TA or IXB. and Cells were treated with TA (1 μM), IXB (1 μM) or ruthenium red (RR; 50 nM) alone, or pretreated with 50 nM of RR 30 min before adding TA or IXB. Ctrl = control cells treated with equal volume of methanol as treated samples. Each value is the mean of  = 15 (100 cells each) ± SD. Statistically different values (t-test followed by Holm-Šídák method,