Development of indirect enzyme-linked immunosorbent assay for detection of porcine epidemic diarrhea virus specific antibodies (IgG) in serum of naturally infected pigs

摘要

Reactivity of pig sera with PED whole virus antigen in indirect ELISA. NT: serum neutralization test; ELISA: Enzyme linked-immunosorbent assay. Dotted line represents cut off value for ELISA test. 81 NT positive and 81 NT negative sera were assesed in our developed indirect ELISA. The sera above dotted line considered PEDV positive and sera below dotted line were PEDV negative according to cut off value for ELISA test