PDIL1-2 can indirectly and negatively regulate expression of the AGPL1 gene in bread wheat

摘要

Qualitative and quantitative detection of the promoter-driven GUS activity in the wheat grains. Qualitative staining observation on GUS driven by three fragments of the TaAGPL1 promoter. Upper panel, Pro-1, Pro-2, and Pro-3 represent 1280 bp (− 1269 bp to + 11 bp), 881 bp (− 870 bp to + 11 bp), and 494 bp (− 483 bp to + 11 bp) of the TaAGPL1 promoter, respectively; 35 S represents CaMV35S promoter; Lower panel, negative controls. Quantitative detection on GUS activities driven by three different fragments of promoter or 35S promoter. Each value is mean ± standard deviation of three biological replicates, and different letters indicate significant differences (