ITR-Seq a next-generation sequencing assay identifies genome-wide DNA editing sites in vivo following adeno-associated viral vector-mediated genome editing

摘要

Sequence analysis of AAV ITRs integrated into genomic DNA. Meta-analysis of on-target AMP-Seq data for all AAV8-M1PCSK9- and AAV8-M2PCSK9-treated liver samples (SRR6343442). Our goal was to identify the most frequent ITR integration start site within the vector ITR. Secondary structure of the AAV2 5′ ITR ( ). The most frequently integrated start site position is shown. The ITR-Seq primer (GSP_ITR3.AAV2) binding site is highlighted in red. A-A’, B-B′, and C-C′, palindromic arms; RBE, Rep-binding element; TRS, terminal resolution site. Schematic diagram of the ITR-Seq protocol used for genome-wide identification of ITR integration sites