Comparison of RNA isolation methods on RNA-Seq: implications for differential expression and meta-analyses

摘要

Schematic of the experimental design. Yeast cells were grown to mid-exponential phase at 30 °C, unstressed control samples were collected, and then cells were shifted to a 37 °C heat shock with samples collected after 20 min. For both unstressed and stressed cells, we collected three identical samples (technical replicates), and RNA was isolated using either hot acid phenol extraction, a Qiagen RNeasy Kit, or a Zymo Research Direct-zol RNA Miniprep Kit. Libraries were constructed in a single batch using a liquid handling robot, and then were pooled and sequenced on a single Illumina HiSeq4000 lane