Scale development and utilization of universal PCR-based and high-throughput KASP markers specific for chromosome arms of rye (Secale cereale L.)

摘要

Genomic in situ hybridization (GISH) and non-denaturing fluorescence in situ hybridization (ND-FISH) analyses of 3RS ditelosomic addition line of ‘Holdfast-KingII’ and 1R disomic addition line of ‘Chinese Spring-Imperial’. For GISH, the rye genomic DNA (green) was used as a probe and Chinese Spring DNA as a blocker. Chromosomes were counterstained with DAPI (blue). GISH analysis of 3RS ditelosomic addition line of ‘Holdfast-KingII’. ND-FISH analysis of the same metaphase cell after GISH analysis ( ) with Oligo-pSc119.2–1 (green) and Oligo-pTa535–2 (red). GISH analysis of 1R disomic addition line of ‘Chinese Spring-Imperial’. ND-FISH analysis of same metaphase cell with after GISH analysis ( ) with Oligo-pSc119.2–1 (green) and Oligo-pAs1–1 (red). The bar represents 10 μm and the arrows represent rye chromosomes or chromosome arms