Inhibitory Activity of Flavonoids Chrysoeriol and Luteolin-7-O-Glucopyranoside on Soluble Epoxide Hydrolase from Capsicum chinense

摘要

Three flavonoids derived from the leaves of Jacq. were identified as chrysoeriol ( ), luteolin-7- -glucopyranoside ( ), and isorhamnetin-7- -glucopyranoside ( ). They had IC values of 11.6±2.9, 14.4±1.5, and 42.7±3.5 µg/mL against soluble epoxide hydrolase (sEH), respectively. The three inhibitors ( – ) were found to non-competitively bind into the allosteric site of the enzyme with values of 10.5 ± 3.2, 11.9 ± 2.8 and 38.0 ± 4.1 µg/mL, respectively. The potential inhibitors and were located at the left edge ofa U-tube shape that contained the enzyme active site. Additionally, we observed changes in several factors involved in the binding of these complexes under 300 K and 1 bar. Finally, it was confirmed that each inhibitor, and , could be complexed with sEH by the “induced fit” and “lock-and-key” models.