Focused ultrasound-induced blood-brain barrier opening improves adult hippocampal neurogenesis and cognitive function in a cholinergic degeneration dementia rat model

摘要

Schematic of the experimental procedure and FUS system. Timeline of the focused ultrasound (FUS) experiment for the analysis of AChE activity and BDNF expression 24 h after sonication (control,  = 4; 192 IgG-saporin [SAP],  = 4; SAP+FUS,  = 4). BrdU and doublecortin (DCX) 5 days after sonication (control,  = 4; SAP,  = 4; SAP+FUS,  = 4). AChE, BDNF, BrdU/NeuN, and BrdU/GFAP 18 days after sonication (control,  = 8; SAP,  = 8; SAP+FUS,  = 8). Confirmed FUS-mediated blood-brain barrier (BBB) opening with MRI. Gadolinium-enhanced T1-weighted images show contrast enhancement. Arrow indicates regions of BBB opening. Confirmed FUS-mediated edema with T2-weighted MRI