Endogenous PCR inhibitors hamper DNA amplification of Mycobacterium tuberculosis DNA in sputum samples. Use of an anion binding resin (GeneReleaser [GR]) improved PCR sensitivity from 77% (27 of 35 culture-positive samples) with phenol-chloroform extraction (P-C) alone to 91% (GR without P-C; P > 0.05) and 100% (P-C and GR combined; P < 0.05).
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机译:内源性PCR抑制剂会阻碍痰标本中结核分枝杆菌DNA的DNA扩增。使用阴离子结合树脂(GeneReleaser [GR])可将PCR灵敏度从仅使用苯酚-氯仿萃取(PC)的77%(35个培养阳性样品中的27个)提高到91%(不使用PC的GR(P> 0.05))和100% %(PC和GR的总和; P <0.05)。
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