Stochastic optical reconstruction microscopy (STORM) provides sub-diffraction-limit image resolution

摘要

We have developed a high-resolution fluorescence microscopy based on high-accuracy localization of photo-switchable fluorophores. In each imaging cycle, only a fraction of fluorophores were turned on, allowing their positions to be determined with nanometer accuracy. The fluorophore positions obtained from a series of imaging cycles were then used to reconstruct the overall image. An imaging resolution of 20 nm was demonstrated and this can in principle reach the molecular scale.