A Rapid Method for Generation of Selective Sox-based Chemosensors of Ser/Thr Kinases Using Combinatorial Peptide Libraries

摘要

A novel screening method to identify selective Sox-based fluorescent probes for Ser/Thr kinases has been developed. Peptide libraries were exposed to a kinase of interest and the products of the timed reaction were analyzed by MALDI-TOF. To demonstrate the potential of this methodology, a selective substrate for Aurora-A kinase was identified that showed a 7-fold improvement in catalytic efficiency over the best substrate described to date in the literature.