BRIEF CONSTANT LIGHT ACCELERATES SEROTONERGIC RE-ENTRAINMENT TO LARGE SHIFTS OF THE DAILY LIGHT-DARK CYCLE

摘要

Brief (~2 day) constant light exposure (LLb) in hamsters dramatically enhances circadian phase-resetting induced by the serotonin (5-HT) receptor agonist, 8-OH-DPAT and other nonphotic stimuli. The present study was undertaken to determine if LLb can also amplify phase-resetting responses to endogenous 5-HT and accelerate re-entrainment to large-magnitude advance and delay shifts of the light-dark (LD) cycle. First, central serotonergic activity was increased by i.p. injection of L-tryptophan +/− the 5-HT reuptake inhibitor fluoxetine. Hamsters under LD or exposed to LLb received vehicle or drugs during the early morning, and phase-shifts of the locomotor activity rhythm were measured after release to constant darkness. Neither drug phase-shifted animals not exposed to LLb (p>0.5 vs. vehicle); however in animals receiving LLb, L-tryptophan with and without fluoxetine produced large phase-advance shifts (means=2.5±0.4h and 2.6±0.2h, respectively; both p<0.035 vs. vehicle). Next, the effects of LLb combined with 8-OH-DPAT or L-tryptophan+fluoxetine on serotonergic re-entrainment to 10 h phase-advance and phase-delay shifts of the LD cycle were assessed. In groups not exposed to LLb, vehicle controls re-entrained slowly to the advance and delay shifts (means=16±1 days and 24±4 days, respectively), but those treated with 8-OH-DPAT re-entrained faster (means=11±2 days and 9±2 days, respectively; both p<0.05 vs. vehicle). In groups exposed to LLb, vehicle controls re-entrained slowly to the advance and delay shifts (means=15±2 and 25±3 days, respectively); however those receiving 8-OH-DPAT rapidly re-entrained to the delay and advance shifts, with the majority (75%) requiring only 1–2 days (means=2±1 days and 4±2 days, respectively; both p<0.05 vs. vehicle). Animals exposed to LLb and treated with L-tryptophan+fluoxetine also exhibited accelerated re-entrainment to a 10 h advance shift (mean=5±2days; p<0.05 vs. vehicle). Thus through enhancing serotonergic phase-resetting, LLb facilitates rapid re-entrainment to large shifts of the LD cycle which offers a potential approach for treating circadian-related desynchronies.